How to Use TLC Silica Plates for Best Results

Categories:Industry News
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Time of issue:2020-08-19
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(Summary description)Thin-layer chromatography silica gel plate, high-efficiency thin-layer chromatography silica gel plate, which is made of high-quality thin-layer chromatography silica gel with appropriate adhesive and spread on glass substrate, with specified pore volume, specific surface and pore distribution . The plate is made of high-purity thin-layer chromatography silica gel (powder) mixed with a certain amount of binder and sprayed. The surface of the plate is pure white, flat, uniform and fine. The main component is SiO2·nH2O, which has stable chemical properties and does not react with other acids and bases except for strong alkali and alkali. Thin-layer chromatography silica gel plates can be directly used for qualitative or quantitative analysis of various types of organic substances.

如何使用薄层层析硅胶板效果最佳

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How to Use TLC Silica Plates for Best Results

(Summary description)Thin-layer chromatography silica gel plate, high-efficiency thin-layer chromatography silica gel plate, which is made of high-quality thin-layer chromatography silica gel with appropriate adhesive and spread on glass substrate, with specified pore volume, specific surface and pore distribution . The plate is made of high-purity thin-layer chromatography silica gel (powder) mixed with a certain amount of binder and sprayed. The surface of the plate is pure white, flat, uniform and fine. The main component is SiO2·nH2O, which has stable chemical properties and does not react with other acids and bases except for strong alkali and alkali. Thin-layer chromatography silica gel plates can be directly used for qualitative or quantitative analysis of various types of organic substances.

Categories:Industry News
Author:
Origin:
Time of issue:2020-08-19
Views:0

Thin-layer chromatography silica gel plate, high-efficiency thin-layer chromatography silica gel plate, which is made of high-quality thin-layer chromatography silica gel with appropriate adhesive and spread on glass substrate, with specified pore volume, specific surface and pore distribution . The plate is made of high-purity thin-layer chromatography silica gel (powder) mixed with a certain amount of binder and sprayed. The surface of the plate is pure white, flat, uniform and fine. The main component is SiO2·nH2O, which has stable chemical properties and does not react with other acids and bases except for strong alkali and alkali. Thin-layer chromatography silica gel plates can be directly used for qualitative or quantitative analysis of various types of organic substances.

Usage of thin layer chromatography silica gel plate

a. The silica gel plate was activated in an oven at 100°C for 30min; after cooling, draw a straight line at 2cm from the bottom end, and use a pencil to lightly mark the spotting position. The distance between the two spotting spots is generally 1-1.5cm;

b. Spotting, the amount of TLC silica gel plate for each spot is generally 0.8μL (depending on the situation), the spot should be as small as possible, if you need to increase the amount, wait for the spot to dry and then place it on the same spot The second and third sampling. Each spotting should be carried out after the last spotting point is dried to avoid excessive diffusion of spotting spots and affect the experimental results;

c. After drying at room temperature, quickly put the sampled silica gel plate into the chromatographic cylinder that has been added with developing agent in advance, seal the chromatographic cylinder, and start the layer development;

d. Take out the silica gel plate when the upper edge of the layer spreading agent is 2 cm away from the upper edge of the silica gel plate for thin layer chromatography, air dry at room temperature and then dry it in an oven at 50°C for 5 minutes, take it out, and put it into a chromatographic cylinder after cooling for the second layer development ;Recommended product: foamed silicone sheet

e. Dry at room temperature after spreading the layer and select the appropriate color development method to develop the color and calculate its Rf value

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How to Use TLC Silica Plates for Best Results

如何使用薄层层析硅胶板效果最佳

How to Use TLC Silica Plates for Best Results

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